Are Involved in Aluminum Response in Flax (Linum usitatissimum L.)05.02.2018 - 782 просмотра
A.A. Dmitriev, A.V. Kudryavtseva, N. L. Bolsheva, A.V. Zyablitsin,
T.A. Rozhmina et. al. miR319, miR390, and miR393
Are Involved in Aluminum Response in Flax (Linum usitatissimum L.).
Bio-Med Research International/ Volume 2017 (2017), Article ID 4975146, 6 pages
Авторы изучали образцы льна на алюмоустойчивость – в нормальных условиях и при воздействии Al+3 в условиях повышенной кислотности в течение 4, 12 и 24 ч.. После чего была проведено секвенирование 16 транскриптомов льна. В результате удалось выявить гены с повышенной и пониженной экспрессией при воздействии Al+3.
На расширенной выборке методом ПЦР показано повышение экспрессии
генов UDP-гликозилтрансфераз, глутатион-S-трансфераз и H+/Ca2+ -обменника CAX3 у устойчивых сортов
Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax (Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390–TAS3 and GRF5, and miR393–AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al response via regulation of growth processes in flax plants.
High-throughput sequencing and qPCR analyses of flax small RNAs allowed us to reveal miRNAs with expression alterations under Al exposure. Our results suggest the involvement of miR319, miR390, and miR393 in Al response in L. usitatissimum plants. Moreover, we revealed diverse alterations of miR390 and miR393 levels in resistant and sensitive to Al genotypes. We concluded that, in flax, potential targets of miR319 are TCPs, miR390–TAS3 and GRF5, and miR393–AFB2. Thus, we speculate that miR319, miR390, and miR393 play an important role in Al stress response via regulation of growth and development processes in flax plants.